Uptake dynamics of nanoagents and effect on radio-enhancement
Project description:
The primary task of my project is the elucidation of the biological impact that nanoagents have on cells. In order to do this, it is necessary to precisely quantify the internalization of nanoagents in different cell types, the type of internalization (endocytosis, pinocytosis, etc.), and the type of cell death that treatment provokes, as well as the effects of the treatment on the genetic material of the cell. Nanoagents achieve all these effects due to high atomic number Z of their constituents (platinum, gold, gadolinium etc.).
In order to achieve all above mentioned, it is necessary to examine different aspects of nanoagents:
1) Quantification of nanoagents' uptake by ICP-MS
2) Localization of the nanoagents in cells by various microscopic techniques (TEM, SEM, Synchrotron radiation-DUV, confocal etc.)
3) Co-localization of nanoagents with cell organelles by utilization of X-ray microscopy. This task will be performed during the secondment at Queen’s University of Belfast.
4) Toxicity studies of nanoagents on cells by performing clonogenic assays
5) Effect of uptaken nanoagents on genetic material of the cell by immunohistochemistry techniques 6. Effect of uptaken nanoagents on cell death by cytoflourimetric techniques
Short CV
Education:
2009-2011: MSc in molecular biology, Faculty of Science, University of Novi Sad, Serbia
2006-2009: BSc in molecular biology, Faculty of Science, University of Novi Sad, Serbia
Work experience:
2013: Early Stage Researcher at University of Calgary, Calgary, Canada and Tsinghua University, Beijing, China
2012: Internship at Scientific Veterinary Institute “Novi Sad”, Novi Sad, Serbia
2011: Internship at Clinical Center of Vojvodina (Immunological Department) in Novi Sad, Serbia